FastQCFastQC Report
Sat 18 Jun 2016
SRR3137932_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137932_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4206580
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG263230.6257577414431675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56500.047.3722761
GTATCAA128700.037.4877051
ATCAACG166600.028.5175063
CAACGCA169500.028.1684025
TATCAAC171700.028.0049532
TCAACGC171050.027.9746534
AACGCAG178000.026.9201876
GTGGTAT19400.026.3469891
ACGCAGA204100.023.2241347
CGCAGAG205400.022.9259858
CGACGAG4600.022.500424
GAGTACT106100.022.24141312
GTACATG208950.021.7182061
GCTCGAT4950.021.60490264
AGAGTAC204150.021.56265611
GATAACG6700.021.1108162
CAGAGTA213900.021.09589610
GCAGAGT220000.020.7616149
GTACTTT109900.020.62576114
TACATGG218350.020.535722
Produced by FastQC (version 0.11.5)