FastQCFastQC Report
Sat 18 Jun 2016
SRR3137932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4206580
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG98230.23351511203875833TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78170.18582791721541014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46070.10951889658582506No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG43120.10250607381768562TruSeq Adapter, Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72250.051.7206541
GTATCAA167350.037.9372181
CTCGTAT28000.028.83163539
TATGCCG28850.028.8192543
ATCAACG223500.028.467993
GCTCGAT5400.028.11061764
TATCAAC226700.028.0357072
TCAACGC228550.027.8842584
TCTCGTA28550.027.79285638
TCGTATG30100.027.6224440
CAACGCA231850.027.5621095
GTGGTAT18350.027.264991
CGACGAG4700.027.15909624
AACGCAG240350.026.7596476
ATGCCGT31550.026.5716444
GATCTCG30300.026.3015236
ATCTCGT31150.025.5838237
TGCGACG5000.025.52985622
CCGTCTT32850.025.20503847
CGTACTA33050.025.0525129
Produced by FastQC (version 0.11.5)