FastQCFastQC Report
Sat 18 Jun 2016
SRR3137926_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137926_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5948014
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG333340.5604223527382417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62350.044.4902271
GTATCAA135500.035.411841
ATCAACG166550.028.2190913
CAACGCA170200.027.8895575
CGACGAG12450.027.4355924
TATCAAC175500.027.1062182
AACGCAG178850.026.7721526
TCAACGC177050.026.7527434
ATGCGAC13650.026.03494621
GCTCGAT13100.025.54366364
TGGTCGT13850.025.1567451
CAATGCG13650.025.02451319
GTACTAG19350.024.4582371
GTCGTCT14800.024.00812553
CGCAGAG201000.023.358698
ACGCAGA203350.023.1396397
CTCGATA14850.022.99807565
GTACGCG902.1290116E-422.9980746
ATCGTCA15500.022.47914110
TGCGACG15300.022.0997222
Produced by FastQC (version 0.11.5)