FastQCFastQC Report
Sat 18 Jun 2016
SRR3137926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5948014
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG71670.1204940001822457RNA PCR Primer, Index 26 (100% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69240.11640860293872879No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG62020.10427009754852629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76350.046.187611
GTATCAA173750.036.5009651
CTCGTAT27050.030.86472139
TATGCCG30250.030.33685343
ATCTCGT28750.029.63942537
ATCAACG222700.027.9204143
CAACGCA225700.027.6565465
TCTCGTA29650.027.46006638
TATCAAC231900.027.0505942
TCAACGC232450.026.7940644
AACGCAG240200.026.2599686
TCGTATG32000.025.6590940
ATGCCGT35300.024.82408744
CGACGAG11000.024.4631624
CCGTCTT35000.023.7552547
TGCCGTC36900.023.74750745
GTGGTAT22300.023.2097281
CGCAGAG265450.023.1604278
ACGCAGA267300.023.1301737
GCTCGAT13550.022.91468264
Produced by FastQC (version 0.11.5)