FastQCFastQC Report
Sat 18 Jun 2016
SRR3137924_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137924_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4800780
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG229740.4785472360741379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39800.039.4951931
GTATCAA79600.033.15861
ATCAACG94850.027.1383343
TCAACGC97550.026.5587984
CAACGCA97450.026.44395
AACGCAG104600.024.7022696
TATCAAC105500.024.607832
CGCAGAG117050.021.9863998
ACGCAGA121150.020.9575797
GTGTAGC30400.020.342081
GATAACG5200.019.9023662
AGAGTAC124050.019.5503411
CAGAGTA135100.018.46180510
GTACATG112150.018.267171
GAGTACT83350.018.21213712
TCGCCCG5350.018.0575522
GCAGAGT139350.017.9728269
GCTCGAT4050.017.88755464
ACATGGG106950.017.7767563
TACATGG114000.017.4734612
Produced by FastQC (version 0.11.5)