FastQCFastQC Report
Sat 18 Jun 2016
SRR3137924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4800780
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG71860.14968400968176004RNA PCR Primer, Index 46 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43950.043.4159851
GTATCAA90000.033.8916851
TATGCCG22750.029.57118643
CTCGTAT21300.029.31635939
TCTCGTA22450.027.81462938
GCGAATC23600.027.48254233
ATCAACG109650.027.4404533
TCAACGC112000.026.8646934
ATGCCGT24850.026.65571844
CAACGCA113250.026.5986395
AATCTCG23350.026.59479536
CGAATCT23750.026.43740334
TCGTATG25500.025.84069640
TATCAAC117900.025.7837162
TGCCGTC25900.025.70828445
CGTATGC26400.025.61316141
CCGTCTT26200.025.15055847
AACGCAG121150.025.0350746
GGCGAAT27950.024.93334832
ATCTCGT26250.024.31383537
Produced by FastQC (version 0.11.5)