FastQCFastQC Report
Sat 18 Jun 2016
SRR3137923_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137923_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5321133
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174290.3275430251414501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48350.041.5137831
GTATCAA108750.036.5008851
CGACGAG2700.029.39008524
ATCAACG132900.029.3639983
TCAACGC133550.029.2414234
CAACGCA137450.028.6624475
TATCAAC140700.027.8238372
AACGCAG143450.027.776236
TGCGACG3000.026.45107722
ACGCAGA163800.024.2200957
CGCAGAG167600.024.0620378
ATGCGAC3600.022.04277221
AGAGTAC173450.022.01756511
CGCACTA3700.021.44460158
GCAGAGT188350.021.0265569
GAGTACT128150.020.99790812
CAGAGTA188000.020.79045310
CAATGCG4050.019.59412819
GTACTTT136950.019.27277814
GTACTAG16700.019.238851
Produced by FastQC (version 0.11.5)