FastQCFastQC Report
Sat 18 Jun 2016
SRR3137923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5321133
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG62840.11809515003665572RNA PCR Primer, Index 33 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55250.045.402741
GTATCAA129600.036.1555331
CTCGTAT17000.030.23792639
TATGCCG18250.029.49038543
ATCAACG158750.028.9517293
TCAACGC159450.028.8462684
AATCTCG17900.028.5245836
CAACGCA162450.028.4410025
TCTCGTA17600.028.42300438
TATCAAC164350.028.2379072
TGCCGTC19600.027.45890445
AACGCAG170300.027.3326246
CCGTCTT19700.027.31951947
ATGCCGT20400.026.38233244
GCCGTCT20950.025.52479746
TCGTATG21850.025.262940
ACGCAGA192300.023.93297
CGCAGAG195800.023.434398
ATCTCGT21950.022.94715937
AGAGTAC205550.021.75194511
Produced by FastQC (version 0.11.5)