FastQCFastQC Report
Sat 18 Jun 2016
SRR3137922_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137922_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250358
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238140.732657756468672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31850.043.2706871
GTATCAA64950.034.3545071
TCAACGC78850.027.7826884
ATCAACG79450.027.5771183
CAACGCA80300.027.4094725
TATCAAC85100.025.8777962
AACGCAG86400.025.5940996
CGCAGAG98650.022.1711258
ACGCAGA101650.021.4489127
AGAGTAC99850.020.73061211
GTACTTT47250.020.15314914
GAGTACT49250.019.96430612
CAGAGTA106150.019.79275510
GCAGAGT111050.019.3229779
AGTACTT52150.018.85469613
GTACATG114950.018.8403471
ACTTTTT53000.018.29220816
TACTTTT51900.018.28104415
CGCGGTA1702.4363162E-618.26371843
TACATGG114750.018.2287162
Produced by FastQC (version 0.11.5)