FastQCFastQC Report
Sat 18 Jun 2016
SRR3137922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250358
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG88160.27123166125085296TruSeq Adapter, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG39980.12300183549012139TruSeq Adapter, Index 10 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33500.045.423411
GTATCAA70300.036.1250421
CTCGTAT25350.033.3426839
TATGCCG26850.033.0218443
TCTCGTA25700.031.41196838
TCGTATG29200.030.83685340
ATGCCGT28950.030.62647444
CCGTCTT29950.029.83426747
TGCCGTC30350.029.66840745
GATCTCG27700.029.26805136
ATCTCGT27700.029.14350737
CGTATGC31400.028.78618241
ATCAACG94550.026.6777043
TCAACGC94750.026.6582184
CAACGCA96300.026.301215
CGTCTTC34750.025.81255348
TATCAAC99550.025.4413812
AACGCAG103600.024.5811716
GTATGCC36400.024.3581442
GCCGTCT37450.023.76732446
Produced by FastQC (version 0.11.5)