FastQCFastQC Report
Sat 18 Jun 2016
SRR3137921_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137921_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6718136
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157570.23454422476710804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45650.041.8445241
GTATCAA93350.033.339931
TCAACGC116350.026.3586754
ATCAACG116400.026.2923853
CAACGCA117750.026.13225
TATCAAC122250.025.1043132
AACGCAG129500.023.8145876
ACGCAGA145650.020.8663837
CGCAGAG144850.020.7196548
AGAGTAC147250.018.86028111
GTACATG152000.018.589341
CAGAGTA159500.017.64916410
TACATGG159500.017.488242
ACATGGG155150.017.0792483
TATACCG2853.2923708E-1016.9455345
GCAGAGT172950.016.5952789
GAGTACT90400.016.29596112
CATGGGG108600.015.5334264
GTGGTAT22950.015.5027511
CGTACGA2705.2670657E-815.33167360
Produced by FastQC (version 0.11.5)