FastQCFastQC Report
Sat 18 Jun 2016
SRR3137921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6718136
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54200.043.2261281
GTATCAA114300.035.2290151
TATGCCG16750.032.33672743
CTCGTAT17200.030.08666439
TCTCGTA16650.029.83729638
ATCAACG144750.027.6519013
TCAACGC147150.027.2245524
CAACGCA148250.027.0462275
TCGTATG20250.026.74766540
ATGCCGT20050.026.6703444
TATCAAC149450.026.6664492
AACGCAG160700.024.9508636
CCGTCTT22700.024.16494247
ATCTCGT20600.024.11606837
CGTATGC22150.023.98602141
TGCCGTC22900.023.50175745
GCCGTCT23250.023.29635246
GATCTCG21300.023.16155236
ACGCAGA180500.021.8287687
CGCAGAG180550.021.7843428
Produced by FastQC (version 0.11.5)