FastQCFastQC Report
Sat 18 Jun 2016
SRR3137920_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137920_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5672985
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG178950.3154423993717593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61650.044.876161
GTATCAA131650.034.7100521
ATGCGAC6600.028.75197821
ATCAACG159300.028.1595863
TCAACGC160950.027.865014
CAACGCA162350.027.7507535
TATCAAC165050.027.398262
CAATGCG5950.027.25445619
AACGCAG171000.026.3066396
AATGCGA6700.024.71811520
ACGCAGA192550.023.2370137
CGCAGAG194400.023.1933358
TGCGACG7350.021.5933222
GCTCGAT5850.021.22904864
CGACGAG7500.021.16145324
CGCACTA7650.020.74341458
AGAGTAC204250.020.60579111
GAGTACT142750.020.49471512
CAGAGTA213150.020.19839910
GCAGAGT219400.020.1102669
Produced by FastQC (version 0.11.5)