FastQCFastQC Report
Sat 18 Jun 2016
SRR3137920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5672985
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG62590.11032992331197772TruSeq Adapter, Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68700.047.560861
GTATCAA153750.035.950411
TATGCCG17100.032.48238843
TCTCGTA17500.030.75367438
CTCGTAT17900.030.45191239
ATCAACG195100.028.1546343
TCAACGC196850.027.904344
TATCAAC196650.027.8795912
CAACGCA199550.027.5447985
GATCTCG20050.026.84236336
AACGCAG209700.026.474836
ATGCCGT21000.026.28565644
GCTCGAT7000.025.62829264
ATCTCGT21350.025.20793237
TGCCGTC21850.025.1052145
TCGTATG22450.024.58770240
CGTATGC22800.024.2102641
GCCGTCT23250.023.74188446
CCGTCTT23650.023.48620847
ACGCAGA234950.023.3338157
Produced by FastQC (version 0.11.5)