FastQCFastQC Report
Sat 18 Jun 2016
SRR3137919_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137919_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6703100
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG191760.2860765914278468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55300.042.538681
GTATCAA126250.035.6239131
TCAACGC154050.028.4408954
CAACGCA155650.028.236565
ATCAACG155350.028.2073143
TATCAAC163300.027.165212
AACGCAG162700.027.0766436
CGACGAG4900.025.34797524
CGCAGAG186050.023.4559148
ACGCAGA187550.023.378687
AGAGTAC196850.020.92553511
GAGTACT120350.020.78320712
CAGAGTA209750.020.09851510
GCAGAGT212850.020.0001379
GTACTTT125800.019.47188814
GTACATG183500.019.20111
ACCGTCG5100.018.94195223
AGTACTT133250.018.79734613
TATATCG5700.018.76196566
TACATGG189350.017.9948392
Produced by FastQC (version 0.11.5)