FastQCFastQC Report
Sat 18 Jun 2016
SRR3137919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6703100
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG69300.10338500096970057RNA PCR Primer, Index 27 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71500.044.346531
GTATCAA157650.035.126211
CTCGTAT21200.028.64120939
TATGCCG22400.027.72292343
TCAACGC199600.027.5710664
CAACGCA199800.027.5607365
ATCAACG200200.027.5229053
TATCAAC205000.026.9624182
AACGCAG210550.026.3502186
TCTCGTA22950.026.30691138
TGCCGTC25550.024.17000245
TATCTCG24650.023.93281236
ATCTCGT25250.023.9106437
CCGTCTT26500.023.17334247
CGCAGAG236850.023.0001018
ACGCAGA239250.022.8274027
ATGCCGT27450.022.49703244
CGACGAG6200.021.70138524
GCCGTCT28550.021.63024746
TCGTATG28900.021.48766540
Produced by FastQC (version 0.11.5)