FastQCFastQC Report
Sat 18 Jun 2016
SRR3137918_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137918_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5292909
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209020.3949057125297261No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG62300.11770464974931556No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG53880.10179657349106135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61650.040.8391151
GTATCAA131700.035.6119581
GCTCGAT15750.030.6648564
TGGTCGT15700.030.32304451
CTCGATA16800.028.5429565
CAATGCG16050.028.37563519
CGACGAG16600.028.05795324
CAACGCA165300.027.9448055
ATCAACG164850.027.8989523
ATGCGAC17950.027.29395521
ATCGTCA17250.027.19864710
TCAACGC172000.026.8765684
TATCAAC174800.026.6187732
AACGCAG176750.026.4077686
GTCGTCT17500.026.21844753
GTACTAG20850.025.840471
CTGGTCG18850.025.62182250
CGCACTA17200.025.27175958
GTGGTAT21200.025.0880371
GCTCGTC20200.024.42228139
Produced by FastQC (version 0.11.5)