FastQCFastQC Report
Sat 18 Jun 2016
SRR3137918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5292909
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70170.13257359988618733No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG69440.1311943961250798No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG57150.1079746506127349No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC55310.10449830140665559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70650.045.1748621
GTATCAA160650.037.2851831
CAATGCG13250.033.58796319
TATGCCG17450.033.01679643
GCTCGAT17400.031.92203564
CTCGATA17700.031.57589365
CTCGTAT17400.031.52548839
TGGTCGT17800.031.2043951
CGACGAG15350.028.99286724
GTCGTCT19150.028.8244553
TCTCGTA19400.028.4532738
ATCGTCA15800.028.3868110
ATCAACG211400.028.2858373
TCGTATG19850.028.15583640
CAACGCA213400.027.9889325
GCTCGTC20250.027.59967239
TATCAAC219300.027.47092
TCAACGC219400.027.3650554
TCGTCTA20650.027.06479854
AACGCAG226650.026.7789656
Produced by FastQC (version 0.11.5)