FastQCFastQC Report
Sat 18 Jun 2016
SRR3137917_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137917_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5776880
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG237760.411571644209331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50550.038.7405851
GTATCAA103800.034.172551
ATCAACG119550.028.8614643
TCAACGC120350.028.6943114
CAACGCA122150.028.2702485
AACGCAG128000.027.1129676
TATCAAC128400.026.9107022
CGCAGAG147400.023.3806748
CGTACGA3250.023.3522360
ACGCAGA147600.023.2087617
ACCGTCG3500.020.70089523
AGAGTAC155400.020.66838311
GAGTACT108450.020.2963412
GCAGAGT168950.019.7654259
CAGAGTA167250.019.53334610
ACTAGCG3550.019.43527455
AATGCGA4950.018.81899620
GTACTTT115800.018.7404514
GCGTACG4050.018.73944359
CGACGAG5000.018.63064624
Produced by FastQC (version 0.11.5)