FastQCFastQC Report
Sat 18 Jun 2016
SRR3137917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5776880
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG90000.1557934386727784RNA PCR Primer, Index 17 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56150.042.2164151
GTATCAA118100.034.738121
TATGCCG25500.030.71141843
CTCGTAT25650.029.05205339
AATCTCG25850.028.69357136
TGCCGTC27450.028.4040545
TCTCGTA26200.028.31050538
ATCAACG141600.028.1700553
TCAACGC142900.027.962084
CAACGCA144550.027.6908825
ATGCCGT28750.027.11969444
TATCAAC149500.026.8880352
ATCTCGT28400.026.48163637
CCGTCTT29550.026.26873847
AACGCAG154400.026.1031236
GCCGTCT29900.025.84586346
TCGTATG30800.024.97839740
CGTCTTC31450.024.35266748
CGTATGC33050.023.69544241
ACGCAGA173750.022.9551987
Produced by FastQC (version 0.11.5)