FastQCFastQC Report
Sat 18 Jun 2016
SRR3137915_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137915_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860251
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG239440.8371293288596001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25650.038.6448551
GTATCAA52650.034.7675931
CAACGCA62850.028.4877285
TCAACGC64350.027.9313894
ATCAACG63650.027.9177573
TATCAAC64900.027.4964752
AACGCAG67250.026.880346
TGCGACG2500.026.22088422
ACGCAGA76300.023.2851187
CGCAGAG77500.023.102638
GAGTACT47450.021.37578412
AGAGTAC80400.020.8104511
CAATGCG3750.020.2413919
CGACGAG3250.020.16991224
AGTACTT50050.019.85281613
GTACTTT49900.019.70507414
CAGAGTA85900.019.63864310
TCGCCGT1601.363971E-619.40520752
GCAGAGT90350.019.0150289
GTACATG72800.018.0755161
Produced by FastQC (version 0.11.5)