Basic Statistics
Measure | Value |
---|---|
Filename | SRR3137915_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2860251 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG | 7920 | 0.27689877566689075 | RNA PCR Primer, Index 46 (96% over 28bp) |
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG | 4009 | 0.14016252419805114 | RNA PCR Primer, Index 46 (96% over 28bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTATCA | 2750 | 0.0 | 44.916077 | 1 |
GTATCAA | 6015 | 0.0 | 36.53888 | 1 |
AATCTCG | 2400 | 0.0 | 30.618355 | 36 |
TATGCCG | 2490 | 0.0 | 30.481535 | 43 |
CTCGTAT | 2435 | 0.0 | 29.894892 | 39 |
GCGAATC | 2575 | 0.0 | 29.475348 | 33 |
TCGTATG | 2655 | 0.0 | 28.847084 | 40 |
CGAATCT | 2625 | 0.0 | 28.782484 | 34 |
TCTCGTA | 2540 | 0.0 | 28.659079 | 38 |
ATCTCGT | 2550 | 0.0 | 28.546688 | 37 |
CGTATGC | 2680 | 0.0 | 28.44926 | 41 |
CAACGCA | 7715 | 0.0 | 28.13026 | 5 |
ATCAACG | 7760 | 0.0 | 27.833746 | 3 |
ATGCCGT | 2740 | 0.0 | 27.826283 | 44 |
TATCAAC | 7830 | 0.0 | 27.760689 | 2 |
TGCCGTC | 2700 | 0.0 | 27.727419 | 45 |
TCAACGC | 7790 | 0.0 | 27.682262 | 4 |
GGCGAAT | 2970 | 0.0 | 27.413788 | 32 |
CCGTCTT | 2685 | 0.0 | 27.36836 | 47 |
AACGCAG | 8130 | 0.0 | 26.907005 | 6 |