FastQCFastQC Report
Sat 18 Jun 2016
SRR3137915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860251
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG79200.27689877566689075RNA PCR Primer, Index 46 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG40090.14016252419805114RNA PCR Primer, Index 46 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27500.044.9160771
GTATCAA60150.036.538881
AATCTCG24000.030.61835536
TATGCCG24900.030.48153543
CTCGTAT24350.029.89489239
GCGAATC25750.029.47534833
TCGTATG26550.028.84708440
CGAATCT26250.028.78248434
TCTCGTA25400.028.65907938
ATCTCGT25500.028.54668837
CGTATGC26800.028.4492641
CAACGCA77150.028.130265
ATCAACG77600.027.8337463
ATGCCGT27400.027.82628344
TATCAAC78300.027.7606892
TGCCGTC27000.027.72741945
TCAACGC77900.027.6822624
GGCGAAT29700.027.41378832
CCGTCTT26850.027.3683647
AACGCAG81300.026.9070056