FastQCFastQC Report
Sat 18 Jun 2016
SRR3137912_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137912_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5633070
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG300540.5335278986414158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55750.046.2189981
GTATCAA120700.034.9123571
ATCAACG144350.028.494293
TCAACGC144750.028.4092454
CAACGCA146050.028.2498535
AACGCAG155400.026.6833296
TATCAAC158550.026.019832
CGCAGAG176950.023.4921678
ACGCAGA179900.022.876837
GAGTACT124950.021.37124412
AGAGTAC183450.021.26955811
CAGAGTA191250.020.8166210
CGACTAT3650.020.79388836
GCAGAGT196200.020.6775349
GTACTTT127500.020.45748114
AGTACTT133550.020.17640713
GATAACG16700.019.6242862
GTGTAGC35050.018.4280831
GCGACTA3800.018.15750135
ACTGACC24900.017.7336148
Produced by FastQC (version 0.11.5)