FastQCFastQC Report
Sat 18 Jun 2016
SRR3137912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5633070
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG117580.20873165076947384TruSeq Adapter, Index 1 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63450.046.54811
GTATCAA141900.036.667291
TATGCCG31250.034.22386643
CTCGTAT31050.033.66623739
TCTCGTA30800.033.04340738
CCGTCTT33700.031.63312747
TGCCGTC34150.031.51964445
ATCTCGT33550.030.84908537
GATCTCG33400.030.67775236
ATGCCGT34800.030.63350344
GCCGTCT36000.029.99571846
TCGTATG36350.029.61199240
ATCAACG181700.028.5409053
CGTCTTC37150.028.50973148
TCAACGC183000.028.3575124
CAACGCA184950.028.1704645
CGTATGC39150.027.49414641
AACGCAG193300.027.0428316
TATCAAC194600.026.808272
GATAACG16100.024.64276362
Produced by FastQC (version 0.11.5)