FastQCFastQC Report
Sat 18 Jun 2016
SRR3137911_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137911_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5811181
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG216760.37300507418371587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55600.042.9252741
GTATCAA116300.035.429951
ATCAACG136150.029.8284823
TCAACGC136950.029.7000274
CAACGCA140050.029.1160145
AACGCAG146450.028.0553876
TATCAAC147200.027.6019122
CGCAGAG165500.024.9925928
ACGCAGA165800.024.7184967
AGAGTAC169900.022.7422511
CGATCGT2005.2750693E-1122.42543426
GAGTACT127500.022.3237712
CAGAGTA183350.021.75076510
GCAGAGT185150.021.7254459
CCGATCG1959.349606E-1021.2311825
GTACTTT135050.020.33595814
AGTACTT139300.019.88872113
GTGTAGC40600.018.3754331
ACTTTTT160900.017.45478616
TAGCCCT48350.017.3386344
Produced by FastQC (version 0.11.5)