FastQCFastQC Report
Sat 18 Jun 2016
SRR3137911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5811181
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG79140.13618574262271302TruSeq Adapter, Index 3 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59350.043.312871
GTATCAA135050.036.536161
TATGCCG22650.030.46296743
CTCGTAT21900.030.08895939
AATCTCG22150.029.749136
ATCAACG163900.029.5157783
TCAACGC165400.029.2689634
CAACGCA168600.028.7551165
TCTCGTA23500.028.18715738
TATCAAC172850.028.1469382
AACGCAG173800.028.0536126
ATGCCGT24550.027.96481944
TGCCGTC24500.027.74002545
CCGTCTT24450.027.37345347
GCCGTCT24850.026.9328346
ATCTCGT26300.025.31742137
ACGCAGA195650.024.688367
TCGTATG28050.024.47586840
CGCAGAG197200.024.3889128
TGCGACG1953.8198777E-1122.99934422
Produced by FastQC (version 0.11.5)