FastQCFastQC Report
Sat 18 Jun 2016
SRR3137910_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137910_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4763024
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG134040.2814178555472322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49550.042.734581
GTATCAA104350.035.089411
ATCAACG124700.028.8311583
TCAACGC125250.028.6455484
CAACGCA127300.028.3462545
AACGCAG131600.027.6559756
TATCAAC132450.027.1842042
CGCAGAG152600.024.098788
ACGCAGA152200.024.071457
TGCGACG3500.022.67217622
ATGCGAC3850.022.40357221
AGAGTAC157650.022.14539711
GAGTACT116600.021.86558312
CGACGAG3700.021.44620324
CAGAGTA169150.021.14945410
GCAGAGT170700.021.1392979
AGTACTT122900.020.7453813
GTACTTT120700.020.63801614
CAATGCG3850.020.61193719
AATGCGA4300.020.05901320
Produced by FastQC (version 0.11.5)