FastQCFastQC Report
Sat 18 Jun 2016
SRR3137910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4763024
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54600.042.531851
GTATCAA119050.036.578151
TATGCCG13900.033.75467343
CTCGTAT13150.033.05597739
AATCTCG13800.031.24900236
TCTCGTA14100.030.33945738
TGCCGTC15850.029.38422645
TCAACGC149150.028.641074
CCGTCTT16300.028.57330747
ATCAACG149500.028.5047763
ATGCCGT16800.028.13332644
CAACGCA151950.028.045175
TATCAAC154400.027.7565942
TCGTATG17050.027.3161340
AACGCAG158150.027.0984386
ATCTCGT16850.025.59265537
GCCGTCT18600.024.66882746
ACCCGCG2053.6379788E-1223.5614615
CGCAGAG179850.023.421768
ACGCAGA181200.023.4008087
Produced by FastQC (version 0.11.5)