FastQCFastQC Report
Sat 18 Jun 2016
SRR3137909_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137909_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4306905
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG306000.7104869970431203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41100.041.7791821
GTATCAA89500.035.2832451
ATCAACG106300.029.084393
CAACGCA109900.028.2824175
TCAACGC109700.028.1456244
ACGTGCG2250.027.5981149
TATCAAC112700.027.5389882
AACGCAG115300.027.1373486
TGCGCGA1850.026.1078412
TGCGACG2850.025.42226822
CGACGAG3000.025.30062124
ACGCAGA129150.024.0401697
CGCAGAG130650.023.8169738
ATCGACG750.001992141623.0035023
GCGCGAT2005.2750693E-1122.42555213
GAGTACT97750.022.09389112
AGAGTAC133600.021.8711411
GCTCGAT4900.021.82503964
CAGAGTA140350.021.31086710
GCAGAGT142850.021.1791619
Produced by FastQC (version 0.11.5)