FastQCFastQC Report
Sat 18 Jun 2016
SRR3137909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4306905
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG111480.2588401648051211TruSeq Adapter, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG51370.11927358509184668TruSeq Adapter, Index 10 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47200.044.7360041
GTATCAA112500.038.8878971
TATGCCG32150.032.836243
CTCGTAT30500.032.124139
TCGTATG34700.030.42316640
TCTCGTA31750.029.99009338
ATCAACG144200.029.9329763
ATGCCGT35700.029.57097644
CCGTCTT35550.029.5016647
CAACGCA147250.029.4776745
TGCCGTC35900.029.40623545
TCAACGC148200.029.195594
TATCAAC149250.028.8732622
GCCGTCT36500.028.63928446
GATCTCG33250.028.53339836
AACGCAG153450.028.4440576
CGTCTTC37700.028.18557248
ATCTCGT33700.028.05001437
CGTATGC38600.027.438741
ACGCAGA170900.025.1959277
Produced by FastQC (version 0.11.5)