FastQCFastQC Report
Sat 18 Jun 2016
SRR3137908_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137908_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4346105
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG359590.8273845201623062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38650.044.0639881
GTATCAA88850.035.8864671
TCAACGC111150.028.1820684
CAACGCA111850.028.128745
ATCAACG111300.028.116973
TATCAAC116700.027.0966722
AACGCAG116650.027.0895736
CGATCGG1401.3911631E-824.64128961
CGCAGAG130200.024.429328
ACGCAGA129900.024.1404957
ACGATCG1502.8936483E-822.99853560
AGAGTAC131000.022.35876711
GAGTACT96500.022.0224112
CAGAGTA142800.021.0905310
GCAGAGT150150.020.5401529
AGTACTT104200.020.16396713
GTACTTT103350.019.9630614
GTACATG92050.018.9144251
ACATGGG89150.018.1126043
TACATGG93800.017.7760662
Produced by FastQC (version 0.11.5)