FastQCFastQC Report
Sat 18 Jun 2016
SRR3137908_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137908_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4346105
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG148870.34253659311038276RNA PCR Primer, Index 39 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG66610.1532636694235413RNA PCR Primer, Index 39 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46120.10611800681299692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51650.048.2992551
GTATCAA119000.036.069981
TATGCCG37350.035.007543
CTCGTAT36750.034.9219239
TCTCGTA36950.034.26605638
CCGTCTT39250.033.04918347
ATGCCGT39700.032.9352744
TGCCGTC40400.032.53539745
GCCGTCT40800.032.13186646
ATCTCGT40050.031.78603637
TCGTATG42400.031.08207740
CGTCTTC42600.030.45024348
CATCTCG41500.030.42604436
CGTATGC44550.029.50460241
ATCAACG156300.027.5065733
GTATGCC47700.027.4838642
TCAACGC157150.027.3358384
TATCAAC158400.027.0326772
CAACGCA159300.026.9669025
AACGCAG164700.026.1036916
Produced by FastQC (version 0.11.5)