FastQCFastQC Report
Sat 18 Jun 2016
SRR3137907_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137907_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5490220
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG254590.46371547952541065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50700.043.3337751
GTATCAA108800.036.0366481
TCAACGC132950.028.9584544
ATCAACG133500.028.8684123
CAACGCA136100.028.3384025
AACGCAG142050.027.3699686
TATCAAC143450.026.6643832
CGCAGAG160550.024.3021058
ACGCAGA162100.023.9846047
GATAACG9450.021.90337862
GAGTACT115750.021.81712512
AGAGTAC169550.021.69036511
CGTTCCG1304.9301107E-621.22942568
GCAGAGT182200.020.9410489
CAGAGTA181100.020.78269610
AGTACTT129050.019.78394113
GTACTTT126350.019.44230714
GTATAGG17400.019.0589681
GTGTAGC31900.018.6258091
GTACATG129000.017.8880961
Produced by FastQC (version 0.11.5)