FastQCFastQC Report
Sat 18 Jun 2016
SRR3137907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5490220
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG98230.17891814899949365RNA PCR Primer, Index 39 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60200.044.134151
GTATCAA133350.038.1145021
CTCGTAT24900.033.52967539
TATGCCG25900.033.1675143
TCTCGTA25050.032.6402838
TGCCGTC27950.030.48796545
TCGTATG28550.030.20975540
ATCTCGT27950.029.62393237
ATGCCGT28850.029.53686744
CCGTCTT29450.029.4036847
ATCAACG172250.029.2264863
TCAACGC172300.029.1584594
CAACGCA174650.028.7661195
TATCAAC179750.028.1605932
GCCGTCT30650.027.91479946
AACGCAG182650.027.7142336
CGTATGC32950.026.3850841
CGTCTTC32900.026.32031848
CATCTCG31900.025.7394736
CGCAGAG206350.024.1927538
Produced by FastQC (version 0.11.5)