FastQCFastQC Report
Sat 18 Jun 2016
SRR3137906_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137906_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5758663
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG268320.46594148676524394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53550.040.8938451
GTATCAA112850.034.3415151
CGACGAG7200.033.0636224
ATCAACG133150.028.167523
CAATGCG8250.028.01961919
CAACGCA134350.027.988365
TCAACGC135250.027.8538554
AACGCAG141200.026.8504526
GCTCGAT8050.026.56956164
TATCAAC145900.025.980352
TGCGACG9000.025.68420822
ATGCGAC9750.025.1239321
CTCGATA8900.024.41963465
GCTCGTC9500.022.87813839
TATATCG8150.022.8572966
CGCAGAG163550.022.8015178
AATGCGA10900.022.78976620
ACGCAGA166950.022.3784837
GTCGTCT10100.021.51847653
ATCGTCA11550.021.2066710
Produced by FastQC (version 0.11.5)