FastQCFastQC Report
Sat 18 Jun 2016
SRR3137906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5758663
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG101660.17653403229187054TruSeq Adapter, Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59100.044.9535331
GTATCAA128900.035.600761
TATGCCG27450.033.3060143
CTCGTAT26350.032.2084539
CAATGCG8200.031.13360419
TGCGACG8400.030.39232822
TCTCGTA27750.029.83757838
ATCTCGT28200.029.72820737
CGACGAG8650.029.51393524
TGCCGTC31200.029.41320445
ATGCCGT31650.029.10426544
GATCTCG29250.028.66104136
CCGTCTT32200.028.17832847
ATCAACG162700.027.4626713
AATGCGA9700.027.38612220
CAACGCA163700.027.3375385
TCAACGC166100.026.8802154
GCTCGAT9250.026.8538264
GCCGTCT34250.026.39124146
TCGTATG35200.026.07102440
Produced by FastQC (version 0.11.5)