FastQCFastQC Report
Sat 18 Jun 2016
SRR3137903_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137903_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4765557
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG780141.637038440627192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40200.040.727231
GTATCAA79350.033.648461
ATCAACG96000.027.0288663
CAACGCA98900.026.1961965
TCAACGC99650.026.138064
TATCAAC104000.025.4939442
AACGCAG104300.025.1042656
ACGCAGA114950.022.4184827
CGCAGAG113200.022.3993578
ATCGACG1154.601467E-521.0030023
ACGCGAA3000.020.69970134
CGCGGTA850.00412340220.29297343
AGAGTAC116800.020.08511411
ATGCGAC4850.019.91807421
GAGTACT79800.019.62774312
CAGAGTA122600.019.6130910
CGACGAG4650.019.29065124
TGACGCG3450.018.99992632
GCAGAGT129600.018.9792549
CGGATCG3300.018.81850224
Produced by FastQC (version 0.11.5)