FastQCFastQC Report
Sat 18 Jun 2016
SRR3137903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4765557
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG194650.40845172977681304RNA PCR Primer, Index 26 (100% over 29bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG92180.1934296452649711RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG54220.11377473818905114RNA PCR Primer, Index 26 (100% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47100.044.1753351
GTATCAA97850.034.7695851
CTCGTAT60300.033.06939
TCTCGTA60250.032.7528838
ATCTCGT65450.031.78470437
TCGTATG62750.031.5579440
TATGCCG66550.031.41485843
ATGCCGT66300.030.96092644
CATCTCG69400.030.77100436
TGCCGTC66150.030.40529645
CGTATGC69300.029.91932141
CCGTCTT65200.029.63131747
GCCGTCT64550.029.44868346
CGTCTTC62650.028.85497548
ATCAACG123150.027.5984863
GTATGCC77550.027.58166142
CAACGCA125300.027.207545
TCAACGC126450.026.878244
GCGTGAA10700.026.76115256
AACGCAG131150.026.1517946
Produced by FastQC (version 0.11.5)