FastQCFastQC Report
Sat 18 Jun 2016
SRR3137902_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137902_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3609527
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG303290.8402485976694454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33600.043.5973131
GTATCAA72050.036.3949281
ATCAACG90250.028.4421733
TCAACGC92400.027.7033884
CAACGCA92950.027.5758155
TATCAAC94650.027.4290732
AACGCAG96800.026.7641536
ACGCAGA110700.022.873757
CGCAGAG111550.022.8540828
GAGTACT73100.021.23802612
AGAGTAC115800.021.003311
GCAGAGT124850.020.1155419
CAGAGTA124750.020.04870810
GTACATG98450.019.792311
AGTACTT81750.019.20183213
TACATGG100000.019.0569322
GTACTTT81350.018.91482514
CGCCGTA1651.8317842E-618.81687453
ACATGGG97450.018.5872233
CATGGGG65000.016.8777564
Produced by FastQC (version 0.11.5)