FastQCFastQC Report
Sat 18 Jun 2016
SRR3137902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3609527
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG121590.3368585412991785RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG55470.15367664516708143RNA PCR Primer, Index 17 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.046.618381
GTATCAA94800.036.5074771
TATGCCG32200.033.10698343
CTCGTAT30650.031.96721839
TCTCGTA31050.031.55540538
AATCTCG31250.031.3525836
ATGCCGT34300.031.18060944
TCGTATG34450.030.94469840
CGTATGC35350.030.15685541
CCGTCTT35150.030.03357947
TGCCGTC35500.029.83465645
GCCGTCT35650.029.41880446
ATCTCGT33450.029.3940737
ATCAACG122450.028.376963
CGTCTTC37150.028.13810248
CAACGCA124750.027.9651955
TATCAAC124150.027.9046252
TCAACGC125150.027.7655264
GTATGCC39550.027.1288242
AACGCAG129200.027.0554126
Produced by FastQC (version 0.11.5)