FastQCFastQC Report
Sat 18 Jun 2016
SRR3137901_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137901_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4389388
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG338860.7719982831319537No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC56060.1277171213845757No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAG49960.11381996761279706No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTT45850.10445647548131996No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG45370.10336292895501605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56750.042.980561
CAATGCG8900.033.7270419
GTATCAA120050.033.642291
GCTCGAT11450.033.44314264
ACGCGTC450.00462537430.66493638
CGACGAG9600.030.54793424
TGGTCGT12550.030.23699251
GCTCGTC13950.028.19195739
GTCGTCT13600.028.15617853
ATCGTCA10900.027.85195710
CTCGATA14050.027.2543865
ATCAACG147900.026.5029263
CAACGCA148300.026.4728455
TGCGACG11400.025.72516322
AACGCAG155700.025.5021256
TATCAAC156300.025.4451392
CGTCATT15400.025.31552526
TCAACGC155750.025.1179684
GTACTAG15800.023.8343141
GTCGTCA16650.023.82965524
Produced by FastQC (version 0.11.5)