FastQCFastQC Report
Sat 18 Jun 2016
SRR3137901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4389388
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG139900.3187232479789893RNA PCR Primer, Index 17 (96% over 28bp)
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC58570.13343545842837315No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG58230.13266086297224125RNA PCR Primer, Index 17 (96% over 28bp)
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAG54620.12443648180566402No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51550.11744234048117869No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG49670.11315928325315511No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTT46550.1060512308321798No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGCGTTCTATCATTCTAGCCTCG45180.10293006678835409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62200.045.2120631
GTATCAA136100.035.2913441
GCTCGAT12550.033.8118864
CAATGCG10300.032.82439419
TATGCCG34000.032.36828243
CTCGTAT32900.032.0873239
AATCTCG33100.031.78921136
TGGTCGT13350.031.52727751
CGACGAG10850.031.16048824
TCTCGTA34200.030.86762638
ATCTCGT35100.029.9778637
CTCGATA14800.028.67155865
ACCGTCG7950.028.20682123
GCTCGTC15300.028.18554939
TCGTATG39550.027.91331540
TGCGACG12250.027.88091522
ATCGTCA12150.027.82802810
CAACGCA174000.027.2287795
TGCCGTC40650.027.15797245
ATGCCGT40800.027.14268544