SRR3137901_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3137901_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4389388
Sequences flagged as poor quality	0
Sequence length	75
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	13990	0.3187232479789893	RNA PCR Primer, Index 17 (96% over 28bp)
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC	5857	0.13343545842837315	No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	5823	0.13266086297224125	RNA PCR Primer, Index 17 (96% over 28bp)
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAG	5462	0.12443648180566402	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5155	0.11744234048117869	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG	4967	0.11315928325315511	No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTT	4655	0.1060512308321798	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGCGTTCTATCATTCTAGCCTCG	4518	0.10293006678835409	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6220	45.212063	1
GTATCAA	13610	35.291344	1
GCTCGAT	1255	33.81188	64
CAATGCG	1030	32.824394	19
TATGCCG	3400	32.368282	43
CTCGTAT	3290	32.08732	39
AATCTCG	3310	31.789211	36
TGGTCGT	1335	31.527277	51
CGACGAG	1085	31.160488	24
TCTCGTA	3420	30.867626	38
ATCTCGT	3510	29.97786	37
CTCGATA	1480	28.671558	65
ACCGTCG	795	28.206821	23
GCTCGTC	1530	28.185549	39
TCGTATG	3955	27.913315	40
TGCGACG	1225	27.880915	22
ATCGTCA	1215	27.828028	10
CAACGCA	17400	27.228779	5
TGCCGTC	4065	27.157972	45
ATGCCGT	4080	27.142685	44