FastQCFastQC Report
Sat 18 Jun 2016
SRR3137900_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137900_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5494529
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG491960.8953633696355048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49650.040.2153171
GTATCAA98650.032.7414131
CAACGCA116050.026.9017935
ATCAACG116500.026.5968973
TCAACGC120050.026.006154
AACGCAG124750.025.3022046
CGACGAG7700.025.09309224
TATCAAC126300.024.9023762
ACCGTCG8000.022.42694923
CGCAGAG142300.022.1093358
ACGCAGA140550.022.0653387
GCTCGAT7250.021.88757564
CGCTACG953.0696348E-421.78740168
TTAGTCG5750.021.59814339
TAGTCGG6100.020.92424240
CAATGCG9250.020.88925419
ATCGTCA9450.020.80792410
TGCGACG9300.020.77618822
GGCGTCG9650.020.376678
CCGTCGG9150.019.98534424
Produced by FastQC (version 0.11.5)