FastQCFastQC Report
Sat 18 Jun 2016
SRR3137900_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137900_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5494529
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG187060.34044774356455304TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG86550.15752032612804484TruSeq Adapter, Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53950.044.7021641
GTATCAA115750.036.1263771
CGACGAG7300.034.9719724
CTCGTAT48700.033.93295739
TATGCCG50350.033.4376343
TCTCGTA50000.032.70570438
GATCTCG50950.032.63758536
ATCTCGT51500.032.1550537
CAATGCG8200.031.1335819
ATGCCGT54450.031.10991144
TCGTATG54900.030.9805940
CCGTCTT54900.030.79206847
TGCCGTC56200.030.01841245
GCCGTCT57450.029.24516746
CGTCTTC59050.028.56959548
CGTACTA59700.028.37437229
ATCAACG147800.028.0123583
CAACGCA148100.027.956125
CGTATGC61350.027.72346541
TCAACGC152050.027.2069284
Produced by FastQC (version 0.11.5)