FastQCFastQC Report
Sat 18 Jun 2016
SRR3137899_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137899_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5088108
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG579181.1383013096420123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50900.044.8625681
GTATCAA102750.034.428541
CAACGCA132200.026.2513715
TATCAAC134100.026.2082162
TCAACGC136200.025.7590184
ATCAACG139000.025.045033
GTACTAG16200.024.7367951
AACGCAG143150.024.5084066
CGACGAG11750.022.02281824
ATCGTCA12100.021.95327410
AATGCGA12450.021.61596920
CAATGCG11700.021.52756919
CGCAGAG157000.021.5116028
ACGCAGA159300.021.4823287
GCTCGAT9500.021.42465664
ATGCGAC14300.020.50842921
TATCGAC11350.020.36726216
GTACATG197600.019.4235021
TCGGGAC11950.019.34292834
CGTACGA10150.019.03300960
Produced by FastQC (version 0.11.5)