FastQCFastQC Report
Sat 18 Jun 2016
SRR3137899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5088108
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG236180.46418039868650585TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG100530.19757835328967072TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG57210.11243865106636887RNA PCR Primer, Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54500.046.5344051
GTATCAA116700.037.166161
TATGCCG60250.033.4400243
CTCGTAT59600.032.58913839
TCTCGTA59650.032.09913338
TCGTATG64800.031.09199340
ATGCCGT65400.030.91255444
TGCCGTC66650.030.48808545
CCGTCTT66550.030.27499447
ATCTCGT63250.030.16305737
CGTATGC68500.029.56395541
GATCTCG64950.029.26733436
ACTAGAT69800.028.61736532
TAGATCT68200.027.92321834
TATCAAC155650.027.8878362
CGTACTA73400.027.3547929
CAACGCA158800.027.2050765
ATCGTCA10750.026.95841610
CAATGCG10750.026.95735519
TACTAGA74650.026.94295531
Produced by FastQC (version 0.11.5)