FastQCFastQC Report
Sat 18 Jun 2016
SRR3137898_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137898_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3666931
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG810582.210513369354373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37200.040.0292551
GTATCAA78800.035.1634371
CAATGCG6550.027.91804919
ATCAACG97650.027.5964363
CAACGCA97400.027.5203135
TATCAAC100500.027.4109652
TCAACGC100450.026.7880954
AACGCAG102950.026.3047756
CGACGAG7150.024.60849424
GTACTAG11000.023.2424661
GCTCGAT9000.022.99852864
TGCGACG7700.022.85105522
ACGCAGA117600.022.6758447
CGCAGAG118600.022.6009988
TGATCGC7800.022.1142719
GTGGTAT14700.022.0929491
AATGCGA9150.021.8695420
TGGTCGT10150.021.75230251
ATGCGAC10100.021.17888321
CCGTATC3750.021.15864655
Produced by FastQC (version 0.11.5)