FastQCFastQC Report
Sat 18 Jun 2016
SRR3137898_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137898_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3666931
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG285790.7793710871570804RNA PCR Primer, Index 46 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG146360.3991348623685584RNA PCR Primer, Index 46 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG76140.20763957652871023RNA PCR Primer, Index 46 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42400.042.5608831
GTATCAA89000.034.9308851
CAATGCG7200.032.58262619
AATCTCG78250.031.8764236
TGCGACG7500.031.73931122
TATGCCG81850.031.69631843
CTCGTAT77550.031.67435839
TCTCGTA78850.031.24007838
ATCTCGT79350.031.21713837
CGAATCT82050.030.90468434
GCGAATC83400.030.9008333
TCGTATG83400.030.90040840
ATGCCGT84750.030.6524344
CGTATGC86450.030.12947841
TGCCGTC86650.029.58216345
CCGTCTT85300.029.56500647
CGTCTTC85100.029.31017548
GTATGCC90550.028.91764842
GAATCTC87550.028.76618635
GCTCGAT9600.028.74937464
Produced by FastQC (version 0.11.5)