FastQCFastQC Report
Sat 18 Jun 2016
SRR3137897_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137897_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4644257
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210420.4530757018829923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG16350.038.6588331
TACCTGG72750.036.2041932
TACCCGG15850.035.935362
GTACCTG85350.031.7673871
ACCTGGG78550.031.2758663
ACCCGGG17650.028.9329383
TGCGACG5950.026.67430122
TACACGG11950.024.2649122
CGTACGA3250.023.35210660
ATGCGAC6950.022.83627321
ACGGGCG4300.022.463445
CCTGGGG57100.022.2943944
GTACTAG10800.022.0668681
GCGACGA7200.022.04310823
ACGTGCG2950.021.0492789
GCGTACG4500.019.93184759
CGCACTA7300.019.84783658
CGACGAG8300.018.70584124
GCTCGAT6300.018.61765964
CGCGATA3600.018.20971914
Produced by FastQC (version 0.11.5)