FastQCFastQC Report
Sat 18 Jun 2016
SRR3137897_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137897_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4644257
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG77120.16605454866085145TruSeq Adapter, Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG17250.041.6053851
TACCTGG81050.035.8454782
TACCCGG18800.035.605672
GTACCTG90400.033.015891
TATGCCG22350.031.18087843
ACCTGGG86750.030.865093
ACCCGGG20550.030.2228893
CTCGTAT22450.029.35158339
TCGTATG23800.028.9912940
TCTCGTA22950.028.41146338
CGTATGC25850.026.95909541
ATGCCGT26750.026.43897244
TGCCGTC26700.026.22977645
GATCTCG25150.026.20023536
TGCGACG5150.026.12593822
ATCTCGT25050.025.6162237
GCCGTCT27950.024.68641346
CCGTCTT28000.024.5191247
CTCGATA6000.023.57449765
CCTGGGG61450.023.5270464
Produced by FastQC (version 0.11.5)